ea0014p269 | (1) | ECE2007
Gartner Wolfgang
, Vila Greisa
, Daneva Teodora
, Koc-Saral Felic
, Majdic Otto
, Nabokih Anastasia
, Luger Anton
, Wagner Ludwig
Objective: Establishment of rodent in vitro cell systems for the extension of the functional data about the recently cloned neuroendocrine marker secretagogin.Methods: 1. DNA-cloning; 2. Antibody generation; 3. Immunoblotting and Immunohistochemistry; 4. Cell-transfection; 5. Luciferase Reporter Assays; 6. ELISA.Results: 1. We characterized the rat homologue of human secretagogin (rat secretagogin) and demonstrated the homol...